The inventive concept here is a process for the use of the 7-membered ring trans-cycloheptene and Si trans-cycloheptenes in biorthogonal chemistry. It provides the fastest known method for biorthogonal labeling. The chemistry of trans-cycloheptene, relative to its homologs, is less explored. This compound is known to undergo rapid isomerization at room temperature. The inclusion of metal compounds like silicon into the cyclic backbone imparts great stability to these trans-cycloalkenes. Although there are several methods for the production of these compounds, their derivatives are known to be thermally sensitive and moisture sensitive. The invention here describes a novel process for the use of trans-cycloheptenes and Si-trans-cycloheptenes with tetrazines and their ability to undergo stable biorthogonal coupling reactions. The rate constants of these biorthogonal coupling reactions can exceed 107 M-1s-1, thus making sila trans-cycloheptene, the most reactive dienophile reported to date for biorthogonal applications.
is the first process describing the use of the 7-membered ring
trans-cycloheptene and Si trans- cycloheptenes in biorthogonal
- This process represents the fastest rate constants to date for biorthogonal labeling.
This process has applications in several areas of medicinal value like,
- cell imaging kits