The invention is a novel means for the fluorescent detection of tyrosine phosphatase and tyrosine kinase, of interest for therapeutic applications. This approach combines a genetic encodability with the exhibition of large fluorescence changes upon phosphorylation, enabling practical advantages over previous tyrosine phosphatase/kinase detection methods. Additionally, this approach is readily applicable to different kinases and phosphatases through modification of the recognition sequence.
assays, in cell extracts, in tissue extracts, and in living cells
fluorescence changes upon phosphorylation
- Applicable to tyrosine kinase activity and inhibitors, as well as tyrosine phosphatase activity and inhibitor