University of Delaware OEIP
University of Delaware

Detection of Genetically Encodable Kinases and Phosphates

Technology #ud10-35

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Researchers
Dr. Neal Zondlo
The Zondlo Research Group focuses on the design, synthesis and development of small molecules and minimalist polymers with biological activity, the elucidation of fundamental principles of and discovery of effectors of biological interactions, the development of novel, functional proteins, and the development of novel and practical methods of synthesis and enantioselective catalysis.
External Link (www1.udel.edu)
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Goswami Joy
Assistant Director, Technology Transfer Center 3028313547
Patent Protection

Tyrosine kinase-inducible domains

US Patent US 8551723 B2
Publications
Design of an Encodable Tyrosine Kinase-Inducible Domain: Detection of Tyrosine Kinase Activity by Terbium Luminescence
Journal of the American Chemical Society Communications, 2010, 132, 5619–5621 9 5619
Design of a Protein Kinase-Inducible Domain
Journal of the American Chemical Society Communications, 2006, 128, 5590-5591

Technology Description:

The invention is a novel means for the fluorescent detection of tyrosine phosphatase and tyrosine kinase, of interest for therapeutic applications. This approach combines a genetic encodability with the exhibition of large fluorescence changes upon phosphorylation, enabling practical advantages over previous tyrosine phosphatase/kinase detection methods. Additionally, this approach is readily applicable to different kinases and phosphatases through modification of the recognition sequence. 

Benefits: 

  • Applicable for in vitro assays, in cell extracts, in tissue extracts, and in living cells 
  • Genetically encodable method 
  • Large fluorescence changes upon phosphorylation 
  • Applicable to tyrosine kinase activity and inhibitors, as well as tyrosine phosphatase activity and inhibitor