This technology involves the development of an approach to detect the activities of protein kinases. Protein kinases and phosphatases are two classes of enzyme that modify cellular protein which leads to changes in regulation and cellular control. Humans have over 500 kinases and 100 phosphatases. Changes in their activities have been implicated in cancer, Alzheimer’s disease, and heart disease. To detect the activities of kinases, proteins have been developed whose structures depend on action by a certain protein kinase. The approach is designed to be applicable to the specific imaging of the activities of each of the individual protein kinases and phosphatases. The novel breakthrough is usage of phosphoserine and phosphotheronine to mimic glutamic acid to indicate kinase activity.
increase in fluorescence, compared to 25% change seen with existing sensors
encodability and large changes in fluorescence not seen together in current sensors
to detect cancerous and pre-cancerous cells